Roche

LightCycler 480 II

LightCycler 480 II
Image courtesy of Thermo Fisher Scientific

High-throughput 384-well qPCR platform with HRM and endpoint genotyping capabilities.

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Overview

The LightCycler 480 II delivers high-throughput real-time PCR with 96 or 384-well block options. Features 6 detection channels, thermal gradient optimization, and advanced HRM analysis for mutation scanning and genotyping. Ideal for gene expression, SNP genotyping, and pathogen detection in clinical research.

Specifications

Sample Capacity 96-well or 384-well block
Reaction Volume 5–20 μL (384-well), 10–100 μL (96-well)
Multiplexing Up to 6 targets / 6-plex
Excitation Source Xenon lamp
Detection CCD camera with 6 filter sets
Excitation Range 450–615 nm
Emission Range 500–670 nm
Max Block Ramp Rate 4.8°C/sec
Temperature Range 37°C to 99°C
Temperature Accuracy ±0.2°C
Temperature Uniformity ±0.3°C
HRM Resolution 0.1°C
Run Time 40 min for 40 cycles
Software LightCycler 480 Software
Connectivity LAN, USB
Dimensions 65 x 52 x 45 cm (W x D x H)
Weight 45 kg
Downstream Molecular Validation

Molecular Readout
Systems

Luminescence, fluorescence, and post-PCR molecular interpretation for integrated amplification workflows.

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Promega GloMax Detection Systems

Complete Your Workflow

Optimized consumables and reagents for LightCycler 480 II

Plates & Seals

LightCycler 480 Multiwell Plate 96

SKU: 04729692001

Plat sumur putih yang didesain khusus untuk mengoptimalkan refleksi sinyal fluoresens dan menekan crosstalk antar sumur pada pembacaan kamera CCD.

Format: 96-Well Plate
Color: White
Material: High-precision Plastic
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Plates & Seals

LightCycler 480 Multiwell Plate 384

SKU: 04729749001

Memastikan transfer panas yang sangat seragam di seluruh 384 sumur untuk volume reaksi mikro (5–20 µL) dalam aplikasi high-throughput.

Format: 384-Well Plate
Color: White
Reaction Vol: 5–20 µL
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Plates & Seals

LightCycler 480 Sealing Foil

SKU: 04729757001

Perekat transparan optik dengan tingkat autofluoresens ultra-rendah untuk menjamin transmisi cahaya bebas distorsi ke detektor filter.

Type: Optical Adhesive Film
Clarity: Ultra-clear
Application: qPCR Seal
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qPCR Reagents

LightCycler 480 SYBR Green I Master

SKU: 04707516001

Master mix siap pakai pasca-optimasi untuk kuantifikasi gen target tunggal dengan linearitas dinamis yang sangat luas.

Concentration: 2X Mix
Detection: SYBR Green I
Format: Hot-Start PCR ready
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qPCR Reagents

LightCycler 480 Probes Master

SKU: 04707494001

Dioptimalkan khusus untuk assay berbasis probe hidrolisis (TaqMan), mendukung multiplexing hingga 6 target secara simultan.

Application: Probe-based qPCR
Multiplex: Up to 6-plex
Enzyme: Hot-start Taq DNA Polymerase
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qPCR Reagents

LightCycler 480 Genotyping Master

SKU: 04707524001

Formulasi master mix performa tinggi untuk analisis variasi genetik (SNP) menggunakan probe hibridisasi maupun teknik HRM.

Application: SNP Genotyping / HRM
Stability: Enhanced enzyme stability
Component: Includes MgCl2 optimized
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