Bio-Rad

CFX Opus 96 Real-Time PCR System

CFX Opus 96 Real-Time PCR System
Image courtesy of Thermo Fisher Scientific

96-well real-time PCR system with 6-channel detection, uniform thermal performance, and cloud connectivity.

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Overview

The CFX Opus 96 Real-Time PCR System delivers precise, reliable results with 5-channel multiplexing plus FRET. Features uniform thermal performance with Peltier heating, shuttle optical system for consistent measurements, and cloud connectivity via BR.io or CFXX Maestro Software. Includes 6 VeriFlex thermal gradient zones for fast optimization and network storage access for excellent data management.

Specifications

Sample Capacity 96-well plate
Sample Volume 1–50 μL (10–50 μL recommended)
Multiplexing Up to 5 targets per well + FRET
Excitation Source 6 filtered LEDs
Detection 6 filtered photodiodes
Excitation Range 450–684 nm
Emission Range 510–730 nm
Max Ramp Rate 5°C/sec
Average Ramp Rate 3.3°C/sec
Temperature Range 4°C to 100°C
Temperature Accuracy ±0.2°C at 90°C
Temperature Uniformity ±0.3°C well-to-well after 10 sec
Gradient Range 30–100°C
Gradient Span 1–24°C
Lid Temperature 30–110°C
Scan Time 12 sec all channels; 3 sec FAM/SYBR only
Sensitivity Detects 1 copy of target in human genomic DNA
Dynamic Range 10 orders of magnitude
Connectivity Wi-Fi, Ethernet, USB 2.0, Cloud BR.io
Dimensions 33 x 56 x 36 cm (W x D x H)
Weight 22 kg
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Optimized consumables and reagents for CFX Opus 96 Real-Time PCR System

Plates & Tubes

Hard-Shell 96-Well PCR Plates

SKU: HSP9601

Desain rigid shell dua komponen yang mencegah distorsi dimensi plat akibat suhu tinggi lid, memastikan posisi sumur tetap presisi untuk optical shuttle scan.

Type: Full-skirted
Frame: Rigid Polycarbonate
Well Material: Polypropylene
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Plates & Tubes

Microseal B Adhesive Seals

SKU: MSB1001

Segel berperekat jernih optik dengan daya rekat ekstra kuat untuk mencegah evaporasi volume mikro (1–50 µL) dan menjaga stabilitas sinyal fluoresens.

Type: Clear Adhesive Film
Temperature: -40°C to 110°C
Optical: High-transmittance clear
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Plates & Tubes

PCR 8-Tube Strips

SKU: TLS0801

Strip tabung low-profile yang meminimalkan ruang mati udara (dead space) untuk memaksimalkan transfer panas dan homogenitas pembacaan optik.

Volume: 0.2 mL
Profile: Low-profile
Format: 8-Tube Strip
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qPCR Reagents

iTaq Universal SYBR Green Supermix

SKU: 1725121

Formulasi master mix interkalasi siap pakai yang diperkuat pewarna pasif ROX, menjamin efisiensi kuantifikasi gen target tunggal yang sangat linear.

Concentration: 2X Mix
Chemistry: SYBR Green I
Reference Dye: ROX Passive Included
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qPCR Reagents

iTaq Universal Probes Supermix

SKU: 1725131

Dioptimalkan khusus untuk teknologi probe hidrolisis (TaqMan), menghasilkan pemisahan sinyal yang bersih pada sistem multiplexing hingga 5 target.

Application: Probe-based qPCR
Multiplexing: Up to 5-plex targets
Enzyme: Antibody-mediated Hot-Start
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