Illumina

MiSeq System

MiSeq System

Compact benchtop sequencer for targeted and small genome sequencing with 4–56h run time.

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Overview

MiSeq System is Illumina's first benchtop sequencer designed for targeted and small genome applications. Features proven SBS chemistry, 1–25M reads per run, and 0.3–15Gb output. Integrated analysis workflows and push-button operation make it ideal for targeted panels, 16S metagenomics, small whole genomes, and amplicon sequencing. Fastest turnaround for clinical labs.

Specifications

Sequencing Technology Sequencing by Synthesis - SBS
Flow Cell Type Standard single flow cell
Max Reads per Run 25M single reads, 50M paired-end reads
Read Length SE36, SE50, PE150, PE250, PE300
Output per Run 0.3–15 Gb
Run Time 4h for 4M reads 1x36, 56h for 25M reads 2x300
Quality Score >80% bases higher than Q30 at 2x150
Applications Targeted panels, 16S metagenomics, small WGS, amplicon seq, HLA typing
Sample Input 50 pg – 1 μg DNA/RNA
Instrument Control 10" Touchscreen
Dimensions 68.6 x 56.5 x 52.3 cm (W x D x H)
Weight 57 kg
Data Analysis On-board MiSeq Reporter + BaseSpace
Connectivity Ethernet, USB
Power Requirements 100–240 VAC, 50/60 Hz, 750W max

Complete Your Workflow

Optimized consumables and reagents for MiSeq System

Reagent Kits

MiSeq Reagent Kit v2 (300-cycle)

SKU: MS-102-2002

Kit reagen standar untuk menghasilkan hingga 15 juta klaster pembacaan, dioptimalkan untuk sekuensing genom kecil, panel target, dan aplikasi ekspresi gen umum.

Chemistry Version: v2 Chemistry
Maximum Cycles: 300 cycles
Output Capacity: Up to 4.5 - 5.1 Gb
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Reagent Kits

MiSeq Reagent Kit v3 (600-cycle)

SKU: MS-102-3003

Kit dengan siklus terpanjang dan densitas klaster tertinggi (hingga 25 juta pasang pembacaan), menjadi standar emas dunia untuk analisis keanekaragaman mikroba 16S metagenomik.

Chemistry Version: v3 Chemistry
Maximum Cycles: 600 cycles
Output Capacity: Up to 13.2 - 15.0 Gb
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Reagent Kits

MiSeq Reagent Micro Kit v2 (300-cycle)

SKU: MS-103-1002

Versi kit v2 skala ekonomis dengan keluaran jumlah read lebih rendah, ideal untuk verifikasi cepat konstruksi klon plasmid atau sekuensing amplikon skala kecil.

Chemistry Version: v2 Micro Format
Maximum Cycles: 300 cycles
Output Capacity: Up to 1.2 Gb
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Library Prep Kits

Nextera XT DNA Library Prep Kit

SKU: FC-131-1024

Kit penyiapan pustaka ultra-cepat menggunakan teknologi *tagmentasi* enzim transposase untuk memotong sekaligus menempelkan adaptor sekuensing pada input DNA yang sangat minim.

Technology: Transposome-mediated Tagmentation
Sample Input: 1 ng
Hands-on Time: < 90 minutes
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Library Prep Kits

TruSeq Custom Amplicon Low Input

SKU: FC-134-2001

Assay penyiapan library berbasis hibridisasi probe tertarget, dirancang khusus untuk memetakan wilayah ekson kustom dari sampel dengan kualitas DNA rendah.

Application: Targeted Resequencing
Input Quality: FFPE / Low-input Optimized
Multiplex Indexing: Dual Indexing Ready
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Library Prep Kits

AmpliSeq for Illumina Cancer Hotspot Panel v2

SKU: 20019161

Panel amplikon terfokus untuk mengamplifikasi dan mendeteksi variasi mutasi titik (SNV) hotspot pada 50 gen yang paling sering bermutasi di berbagai jenis kanker.

Application: Targeted Oncology Sequencing
Target Genes: 50 Cancer Hotspot Genes
Input DNA: Low-input (10 ng)
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